Technology / DBTL cycle

Our technology combines synthetic biology technology, Information technology, and robotics to build an advanced microbial development platform. By understanding the meaning (function) of the genome sequence of a gene and being able to design it freely, the speed of microbial development will be dramatically improved.

Core technology “DBTL Cycle” Design living organisms from DNA and create smart cells with intended functions

What is a DBTL cycle?

This is a model that cycles through a series of steps in microbial development. It consists of four technologies: Design, Build, Test, and Learn.

<D>Design

In order to maximize the production of target compounds, we design new artificial metabolic pathways and create blueprints for metabolic reactions that living organisms do not originally have.

Setting target compound
目的化合物の設定イメージ
Artificial metabolic reaction
Artificial enzyme design
Artificial metabolic reaction/enzyme included metabolic pathway design
人工代謝反応/人工酵素を含む代謝経路設計イメージ
Modification
simulation
Metabolic pathway
(Optimal design)
代謝経路最適設計イメージ
Bacterial strain creation
Increased production strain
高生産株イメージ

Cheminformatics-based metabolic pathway design tool
Bioinformatics-based enzyme design tool

Simulation-based optimization tool for overall metabolic pathway design

<B>Build

Creating designer microorganisms. Since the number of designer candidates created by “Design tool” is enormous, we will use robotics-based automation to rapidly create designer microorganisms in parallel.

Utilizing mechanization and automation (lab automation) of the “Build” process

<T>Test

We evaluate the functions of the constructed microorganisms by analyzing the internal and external metabolomes. In order to process a huge number of microorganisms, we are building a high-throughput evaluation system using automated mechanization.

Multi-scale bio fermenter

  • 培養装置の写真

    100mL

    培養装置の写真

    1L

  • 培養装置の写真

    5L

    培養装置の写真

    30L

Metabolomic analyzer

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    LCMS

    メタボローム分析用装置の写真

    GCMS

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    メタボローム分析用装置の写真

    HPLC + Fraction collector

<L>Learn

We combine collected microbial evaluation data with statistical analysis to accumulate and analyze data. By “Learn” model, we will derive new rules and lead to better Design.

DBTL CycleBy cycling through DBTL, the productivity can be improved

1st generation:
Explore the pathway of wild strains and produce polymer.
2nd generation:
Enzymatic improvements are made to the wild-type strain to increase the amount of polymer produced.
3rd generation:
Improve and scale up the expression system to further improve polymer production.
4th generation:
Overall optimization and scale-up to further improve polymer production.

One-stop solution from microbial breeding to process developmentIntegrated BioFoundry®

We have comprehensive technology from smart cell development to production process development,
for achieving high-speed commercialization.

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