{"id":503,"date":"2024-05-02T19:48:50","date_gmt":"2024-05-02T10:48:50","guid":{"rendered":"https:\/\/www.b2i.co.jp\/?page_id=503"},"modified":"2024-12-13T11:18:12","modified_gmt":"2024-12-13T02:18:12","slug":"technology","status":"publish","type":"page","link":"https:\/\/www.b2i.co.jp\/en\/technology\/","title":{"rendered":"<b>Technology \/ DBTL cycle<\/b>"},"content":{"rendered":"<div class=\"technology\">\r\n<div class=\"content_box\">\r\n<div class=\"fz_18 text_center_nsp\">\r\n<p>Our technology combines synthetic biology technology, Information technology, and robotics to build an advanced microbial development platform. By understanding the meaning (function) of the genome sequence of a gene and being able to design it freely, the speed of microbial development will be dramatically improved.<\/p>\r\n<\/div>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div class=\"lead mb_m\">\r\n<p>Core technology <b>\u201cDBTL Cycle\u201d<\/b> <span class=\"small small_bottom\">Design living organisms from DNA and create smart cells with intended functions<\/span><\/p>\r\n<\/div>\r\n<figure class=\"technology_dbtl_img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_dbtl_img_en.png\" alt=\"\" width=\"1458\" height=\"758\" class=\"alignnone wp-image-587 size-full\" \/><\/figure>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<h2>What is a DBTL cycle?<\/h2>\r\n<div class=\"mb_ml\">\r\n<p>This is a model that cycles through a series of steps in microbial development. It consists of four technologies: Design, Build, Test, and Learn.<\/p>\r\n<\/div>\r\n<ul class=\"link_list\">\r\n\t<li><a class=\"linkListBtn\" href=\"#design\">\uff1cD\uff1eDesign<\/a><\/li>\r\n\t<li><a class=\"linkListBtn\" href=\"#build\">\uff1cB\uff1eBuild<\/a><\/li>\r\n\t<li><a class=\"linkListBtn\" href=\"#test\">\uff1cT\uff1eTest<\/a><\/li>\r\n\t<li><a class=\"linkListBtn\" href=\"#learn\">\uff1cL\uff1eLearn<\/a><\/li>\r\n<\/ul>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div id=\"design\" class=\"a_point\"><\/div>\r\n<h3 class=\"mt0\">\uff1cD\uff1eDesign<\/h3>\r\n<div class=\"mb_m\">\r\n<p>In order to maximize the production of target compounds, we design new artificial metabolic pathways and create blueprints for metabolic reactions that living organisms do not originally have.<\/p>\r\n<\/div>\r\n<div class=\"technology_design_imgs\">\r\n<div class=\"technology_design_list\">\r\n<div class=\"technology_design_list_img_wrap\">\r\n<div class=\"txt\">Setting target compound<\/div>\r\n<figure class=\"technology_design_list_img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/design_01.png\" alt=\"\u76ee\u7684\u5316\u5408\u7269\u306e\u8a2d\u5b9a\u30a4\u30e1\u30fc\u30b8\" width=\"115\" height=\"129\" class=\"alignnone size-full wp-image-213\" \/><\/figure>\r\n<\/div>\r\n<div class=\"technology_design_list_txt\"><span class=\"arrow\"><\/span> <span class=\"txt\"> Artificial metabolic reaction<br \/>\r\nArtificial enzyme design<\/span><\/div>\r\n<div class=\"technology_design_list_img_wrap\">\r\n<div class=\"txt\">Artificial metabolic reaction\/enzyme included metabolic pathway design<\/div>\r\n<figure class=\"technology_design_list_img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/design_02.png\" alt=\"\u4eba\u5de5\u4ee3\u8b1d\u53cd\u5fdc\uff0f\u4eba\u5de5\u9175\u7d20\u3092\u542b\u3080\u4ee3\u8b1d\u7d4c\u8def\u8a2d\u8a08\u30a4\u30e1\u30fc\u30b8\" width=\"111\" height=\"129\" class=\"alignnone size-full wp-image-214\" \/><\/figure>\r\n<\/div>\r\n<div class=\"technology_design_list_txt\"><span class=\"arrow\"><\/span> <span class=\"txt\"> Modification<br \/>\r\nsimulation<\/span><\/div>\r\n<div class=\"technology_design_list_img_wrap\">\r\n<div class=\"txt\">Metabolic pathway<br \/>\r\n(Optimal design)<\/div>\r\n<figure class=\"technology_design_list_img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/design_03.png\" alt=\"\u4ee3\u8b1d\u7d4c\u8def\u6700\u9069\u8a2d\u8a08\u30a4\u30e1\u30fc\u30b8\" width=\"110\" height=\"129\" class=\"alignnone size-full wp-image-230\" \/><\/figure>\r\n<\/div>\r\n<div class=\"technology_design_list_txt\"><span class=\"arrow\"><\/span> <span class=\"txt\"> Bacterial strain creation <\/span><\/div>\r\n<div class=\"technology_design_list_img_wrap\">\r\n<div class=\"txt\">Increased production strain<\/div>\r\n<figure class=\"technology_design_list_img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/design_04.png\" alt=\"\u9ad8\u751f\u7523\u682a\u30a4\u30e1\u30fc\u30b8\" width=\"110\" height=\"129\" class=\"alignnone size-full wp-image-215\" \/><\/figure>\r\n<\/div>\r\n<\/div>\r\n<div class=\"technology_design_list_bottom\">\r\n<div class=\"technology_design_list_bottom_txt\">\r\n<p>Cheminformatics-based metabolic pathway design tool<br \/>\r\nBioinformatics-based enzyme design tool<\/p>\r\n<\/div>\r\n<div class=\"technology_design_list_bottom_txt\">\r\n<p>Simulation-based optimization tool for overall metabolic pathway design<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div id=\"build\" class=\"a_point\"><\/div>\r\n<h3 class=\"mt0\">\uff1cB\uff1eBuild<\/h3>\r\n<div class=\"mb_m\">\r\n<p>Creating designer microorganisms. Since the number of designer candidates created by \u201cDesign tool\u201d is enormous, we will use robotics-based automation to rapidly create designer microorganisms in parallel.<\/p>\r\n<\/div>\r\n<div class=\"fz_20 fw_700 w_fit mb_ms\">\r\n<p>Utilizing mechanization and automation (lab automation) of the \u201cBuild\u201d process<\/p>\r\n<\/div>\r\n<figure class=\"technology_build_img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_build_img_en.png\" alt=\"\" width=\"1452\" height=\"655\" class=\"alignnone wp-image-589 size-full\" \/><\/figure>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div id=\"test\" class=\"a_point\"><\/div>\r\n<h3 class=\"mt0\">\uff1cT\uff1eTest<\/h3>\r\n<div class=\"mb_m\">\r\n<p>We evaluate the functions of the constructed microorganisms by analyzing the internal and external metabolomes. In order to process a huge number of microorganisms, we are building a high-throughput evaluation system using automated mechanization.<\/p>\r\n<\/div>\r\n<div class=\"columnbox pc2 sp1 business_product_list_wrap\">\r\n<div class=\"column_item\">\r\n<h4 class=\"glay_box_ttl txt_c mb_3\">Multi-scale bio fermenter<\/h4>\r\n<ul class=\"business_product_list technology_test_list\">\r\n\t<li>\r\n<div class=\"business_product_list_item\">\r\n<div class=\"columnbox pc2 pcgap4 pcvgap6\">\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_04.jpg\" alt=\"\u57f9\u990a\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-225\" \/><\/figure>\r\n<div class=\"technology_test_list_cap\">\r\n<p>100mL<\/p>\r\n<\/div>\r\n<\/div>\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_02.jpg\" alt=\"\u57f9\u990a\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-223\" \/><\/figure>\r\n<div class=\"technology_test_list_cap\">\r\n<p>1L<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/li>\r\n\t<li>\r\n<div class=\"business_product_list_item\">\r\n<div class=\"columnbox pc2 pcgap4 pcvgap6\">\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_03.jpg\" alt=\"\u57f9\u990a\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-224\" \/><\/figure>\r\n<div class=\"technology_test_list_cap\">\r\n<p>5L<\/p>\r\n<\/div>\r\n<\/div>\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_30l.jpg\" alt=\"\u57f9\u990a\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone wp-image-420 size-full\" \/><\/figure>\r\n<div class=\"technology_test_list_cap\">\r\n<p>30L<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/li>\r\n<\/ul>\r\n<\/div>\r\n<div class=\"column_item\">\r\n<h4 class=\"glay_box_ttl txt_c mb_3\">Metabolomic analyzer<\/h4>\r\n<ul class=\"business_product_list technology_test_list\">\r\n\t<li>\r\n<div class=\"business_product_list_item\">\r\n<div class=\"columnbox pc2 pcgap4 pcvgap6\">\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_05.jpg\" alt=\"\u30e1\u30bf\u30dc\u30ed\u30fc\u30e0\u5206\u6790\u7528\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-226\" \/><\/figure>\r\n<div class=\"technology_test_list_cap\">\r\n<p>LCMS<\/p>\r\n<\/div>\r\n<\/div>\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_06.jpg\" alt=\"\u30e1\u30bf\u30dc\u30ed\u30fc\u30e0\u5206\u6790\u7528\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-227\" \/><\/figure>\r\n<div class=\"technology_test_list_cap\">\r\n<p>GCMS<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/li>\r\n\t<li>\r\n<div class=\"business_product_list_item\">\r\n<div class=\"columnbox pc2 pcgap4 pcvgap6\">\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_07.jpg\" alt=\"\u30e1\u30bf\u30dc\u30ed\u30fc\u30e0\u5206\u6790\u7528\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-228\" \/><\/figure>\r\n<\/div>\r\n<div class=\"column_item\">\r\n<figure><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_test_img_08.jpg\" alt=\"\u30e1\u30bf\u30dc\u30ed\u30fc\u30e0\u5206\u6790\u7528\u88c5\u7f6e\u306e\u5199\u771f\" width=\"240\" height=\"188\" class=\"alignnone size-full wp-image-229\" \/><\/figure>\r\n<\/div>\r\n<\/div>\r\n<div class=\"technology_test_list_cap\">\r\n<p>HPLC + Fraction collector<\/p>\r\n<\/div>\r\n<\/div>\r\n<\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div id=\"learn\" class=\"a_point\"><\/div>\r\n<h3 class=\"mt0\">\uff1cL\uff1eLearn<\/h3>\r\n<div class=\"mb_m\">\r\n<p>We combine collected microbial evaluation data with statistical analysis to accumulate and analyze data. By \u201cLearn\u201d model, we will derive new rules and lead to better Design.<\/p>\r\n<figure class=\"mt5\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/learn_img_en.png\" alt=\"\" width=\"1675\" height=\"634\" class=\"alignnone wp-image-591 size-full\" srcset=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/learn_img_en.png 1675w, https:\/\/www.b2i.co.jp\/wp-content\/uploads\/learn_img_en-1536x581.png 1536w\" sizes=\"auto, (max-width: 1675px) 100vw, 1675px\" \/><\/figure>\r\n<\/div>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div class=\"glay_box glay_box_ha\">\r\n<h3 class=\"glay_box_ttl mb_ms txt_c\"><span class=\"en\">DBTL Cycle<\/span>By cycling through DBTL, the productivity can be improved<\/h3>\r\n<figure class=\"technology_productivity_img mb_m\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.b2i.co.jp\/wp-content\/uploads\/technology_productivity_img_en02-1.png\" alt=\"\" width=\"1238\" height=\"584\" class=\"alignnone wp-image-657 size-full\" \/><\/figure>\r\n<div class=\"w_fit\">\r\n<dl class=\"business_analysis_sub_list\">\r\n<dt>1st generation:<\/dt>\r\n<dd>Explore the pathway of wild strains and produce polymer.<\/dd>\r\n<\/dl>\r\n<dl class=\"business_analysis_sub_list\">\r\n<dt>2nd generation:<\/dt>\r\n<dd>Enzymatic improvements are made to the wild-type strain to increase the amount of polymer produced.<\/dd>\r\n<\/dl>\r\n<dl class=\"business_analysis_sub_list\">\r\n<dt>3rd generation:<\/dt>\r\n<dd>Improve and scale up the expression system to further improve polymer production.<\/dd>\r\n<\/dl>\r\n<dl class=\"business_analysis_sub_list\">\r\n<dt>4th generation:<\/dt>\r\n<dd>Overall optimization and scale-up to further improve polymer production.<\/dd>\r\n<\/dl>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"content_box\">\r\n<div class=\"bg_box\">\r\n<div class=\"lead text_center mb_ms\">\r\n<h2 class=\"inherit_ttl\"><span class=\"small\">One-stop solution from microbial breeding to process development<\/span>Integrated BioFoundry<\/h2>\r\n<\/div>\r\n<div class=\"text_center_nsp\">\r\n<p>We have comprehensive technology from smart cell development to production process development,<br \/>\r\nfor achieving high-speed commercialization.<\/p>\r\n<\/div>\r\n<a href=\"\/en\/business\/\" class=\"linkBtn linkBtn_center\"> Know more <\/a><\/div>\r\n<\/div>\r\n<\/div>","protected":false},"excerpt":{"rendered":"Our technology combines synthetic biology technology, Information technology, and robotics to build an advance ...","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":300,"comment_status":"closed","ping_status":"closed","template":"page-nosidebar.php","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_locale":"en_US","_original_post":"https:\/\/www.b2i.co.jp\/?page_id=71","footnotes":""},"class_list":["post-503","page","type-page","status-publish","hentry","en-US"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/pages\/503","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/comments?post=503"}],"version-history":[{"count":16,"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/pages\/503\/revisions"}],"predecessor-version":[{"id":781,"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/pages\/503\/revisions\/781"}],"wp:attachment":[{"href":"https:\/\/www.b2i.co.jp\/wp-json\/wp\/v2\/media?parent=503"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}